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Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers

機(jī)譯:通過集成的等離激元納米二聚體增強(qiáng)的通過拉曼光譜進(jìn)行連續(xù)單細(xì)胞分析的微流體裝置

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摘要

In this work a Raman flow cytometer is presented. It consists of a microfluidic device that takes advantages of the basic principles of Raman spectroscopy and flow cytometry. The microfluidic device integrates calibrated microfluidic channels- where the cells can flow one-by-one -, allowing single cell Raman analysis. The microfluidic channel integrates plasmonic nanodimers in a fluidic trapping region. In this way it is possible to perform Enhanced Raman Spectroscopy on single cell. These allow a label-free analysis, providing information about the biochemical content of membrane and cytoplasm of the each cell. Experiments are performed on red blood cells (RBCs), peripheral blood lymphocytes (PBLs) and myelogenous leukemia tumor cells (K562).
機(jī)譯:在這項(xiàng)工作中提出了拉曼流式細(xì)胞儀。它由利用拉曼光譜和流式細(xì)胞儀基本原理的微流控設(shè)備組成。微流控設(shè)備集成了校準(zhǔn)的微流控通道(細(xì)胞可以在其中流過一個通道),從而可以進(jìn)行單細(xì)胞拉曼分析。微流體通道在流體捕獲區(qū)域中整合了等離子體納米二聚體。以此方式,可以在單細(xì)胞上進(jìn)行增強(qiáng)拉曼光譜。這些允許進(jìn)行無標(biāo)記分析,從而提供有關(guān)每個細(xì)胞的膜和細(xì)胞質(zhì)的生化含量的信息。對紅細(xì)胞(RBC),外周血淋巴細(xì)胞(PBL)和骨髓性白血病腫瘤細(xì)胞(K562)進(jìn)行了實(shí)驗(yàn)。

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